castanyes blaves

[...] the addition of GS FLX Paired End reads vastly improved the capability of 454 pyrosequencing by enabling the assembly of contigs into large scaffolds. Indeed, in terms of the number of scaffolds produced, the GS FLX assembly that included the combined shotgun and paired end reads was comparable to the Sanger assembly. Moreover, the order of the GS FLX scaffolds could be established from information from BAC-end sequences and the Atlantic salmon physical map. However, numerous gaps remained within the scaffolds, which is undesirable when a complete or reference genome sequence is one of the goals. Currently, if the Atlantic salmon genome is to provide a reference sequence for all salmonids, then a substantial proportion of the sequencing will have to be carried out using Sanger technology.