castanyes blaves
Random ramblings about some random stuff, and things; but more stuff than things -- all in a mesmerizing and kaleidoscopic soapbox-like flow of words.10/03/2007
Charlie Boone -- Global mapping of genetic and chemical-genetic interactions in yeast
http://en.wikipedia.org/wiki/Epistasis
Synthetic lethality or unlinked non-complementation - two mutations fail to complement and yet do not map to the same locus.
World canavinine crisis... :p
S. pombe biology is better than yeast for high-throughput (HTP) genetic interaction analysis
HTP Quantitative Cell Biology
bni1 synthetic lethality -- a highly complex network of 50 genes
8 SGA screens -- 291 interactions -- 204 genes
1000 genes - ways to kill the cell when ko a gene
100,000 ways when ko two genes
Interactions occur amongst functionally related genes
Genetic Network is a Small World Network
Scoring Genetic Interactions Quantitatively -- linear regression model for colony size -- doublings per hour
syntethic lethals VS pairs of genes whose products are in the same nonessential complex/pathway
Molecular Barcodes Tag Deletion Strains -- barcodes for each mutant: 20bp oligo pairs -- stitching PCR -- drug hypersensitivity screen -- barcode chip
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