Anolis carolinensis: First reptile in Ensembl -- Amonida Sadissa

• Very few anolis proteins and cDNAs, many more ESTs.
• Used Uniprot PE Evidence ranking to generate transcript models with genewise
• Different parameters for Exonerate, including exhaustive option for cDNAs, including 31000 chicken set (which wasn't very useful in the end)
• Chris Ponting group provided extra models and kill list to rename some retrotransposons and pseudogenes
• Manually looked at some of the EST genes in Chris' list to reincorporate them in the main db

Word of advise to all Genome Sequencing Centers out there: now that RNAseq is cheap and powerful, please allocate some of your budget for that instead of spending all in genomic sequencing. Contact Sanger people for PCR-free sample preparation protocols, which makes a huge difference in terms of avoiding duplicity.